Dynamic Biosensors helix cyto Normalization Solution
وضاحتون
- پيداوار جو نالو: heliX cyto Normalization Solution (Red Dye)
- آرڊر نمبر: NOR-R2
- For scIC measurements in the red channel
- صرف تحقيق لاء استعمال ڪريو
- Limited shelf life – please check expiry date on label
اهم خاصيتون
- هيليڪسيٽو چپ جي اسپاٽ 1 ۽ اسپاٽ 2 تي فلورسنٽ سگنلز کي معمول تي آڻڻ لاءِ
- Enables correct real-time referencing of the red fluorescent signals during scIC measurements
- Compatible with all heliXcyto chips
- The Normalization solution (red dye) contains a hydrophilic red dye with a single negative net charge.
پيداوار جي وضاحت
- آرڊر نمبر: NOR-R2
جدول 1. مواد ۽ اسٽوريج جي ڄاڻ
| مواد | ڪپ | Concentr ation | رقم | ذخيرو |
| Normalization solution-R2 | ڳاڙهو | 10 µM | 6x 100 µL | -20 °C |
- صرف تحقيق لاء استعمال ڪريو.
- This product has a limited shelf life; please see the expiry date on the label.
- To avoid many freeze-thaw cycles, please aliquot the solution.
تياري
- Use this red dye normalization solution for scIC measurements in the red channel (analyte label-dependent).
- Dilute the 10 µM normalization stock solution to a working concentration with running buffer.
- The concentration of the normalization solution should approximately correspond to the fluorophore concentration in the highest analyte concentration to be measured. This can be calculated usingthe following equation:
Concentration of the normalization solution in the desired color :
ليبل ٿيل تجزياتي حل ۾ رنگ جي ڪنسنٽريشن
Highest concentration of the analyte that should be measured
Degree of labeling (ratio of dye to analyte)
حل ٿيل حل 2-8 ° C تي 7 ڏينهن تائين محفوظ ڪري سگھجي ٿو.
ايپليڪيشن نوٽ
In the scIC measurement, the fluorescent signal of the normalization solution should be in a similar range to the highest signal coming from the bound analyte (raw data). The absolute fluorescent signal is dependent on normalization solution concentration and the excitation power applied in the measurement. The excitation power has to be selected based on the following parameters:
- Fluorophore concentration inthe analyte solution
فلوروفور ڪنسنٽريشن جو دارومدار ماپ ۾ استعمال ڪيل تجزياتي ڪنسنٽريشن تي آهي ۽ ان سان گڏ تجزيي جي ليبلنگ جي درجي تي. اعليٰ DOL ۽ اعليٰ تجزياتي ڪنسنٽريشن لاءِ، حوصلي واري طاقت کي گھٽائڻ جي ضرورت پوندي. - متوقع پابند سگنل
Highly expressed targets on a cell can bind more molecules of the labeled analyte. In case of highly overexpressed targets, a strong binding signal can be expected. To avoid the shutter closing, lowering the excitation power might be considered. - چپ جو قسم
مختلف چپ جي قسمن ۾ مختلف فلورسنٽ پس منظر آهن. جيترا وڏا ڦڙا ۽ چپ تي وڌيڪ جال، اوترو ئي پس منظر سگنل. تنهن ڪري، L5 چپس شايد M5 چپس تي لاڳو ٿيڻ جي ڀيٽ ۾ گھٽ جوش واري طاقت جي ضرورت هوندي آهي.
For a starting point of excitation power and normSolution on concentration to be used in an scIC experiment, please refer to Table 2.
Table 2. Relation of fluorophore concentration, normalization solution concentration, and excitation power suitable for an 
ايم 5 چپ
| تجزياتي رنگ جي ڪن. = analyte conc x DOL | جوش جي طاقت | ڪنسنٽريشن نارملائيزيشن حل | Dilution Normalization حل |
| 25 ن ايم | 0.5 | 25 ن ايم | 1:400 |
| 50 ن ايم | 0.3 | 50 ن ايم | 1:200 |
| 100 ن ايم | 0.2 | 100 ن ايم | 1:100 |
| 300 ن ايم | 0.1 | 300 ن ايم | 1:33 |
| 500 ن ايم | 0.08 | 500 ن ايم | 1:20 |
| 1 µM | 0.05 | 1 µM | 1:10 |
| 2.5 µM | 0.02 | 2.5 µM | 1:4 |
نوٽ: This table is for your guidance. However, the final signal recorded in the heliXcyto depends on many factors. Thus, some optimization will be required for each system.
رابطو
Dynamic Biosensors GmbH
Perchtinger Str. 8/10 81379 Munich, Germany
بروڪر سائنٽيفڪ ايل ايل سي
40 ميننگ روڊ، ميننگ پارڪ، بلريڪا، ايم اي 01821 آمريڪا
- آرڊر جي ڄاڻ
- ٽيڪنيڪل سپورٽ
www.dynamic-biosensors.com
اوزار ۽ چپس جرمني ۾ انجنيئر ۽ تيار ڪيا ويا آهن. ©2025 ڊائنامڪ بايوسينسر GmbH صرف تحقيق جي استعمال لاءِ. ڪلينڪل تشخيصي طريقيڪار ۾ استعمال لاءِ نه.
FAQs
What is the shelf life of the heliX cyto Normalization Solution?
پيداوار هڪ محدود شيلف زندگي آهي. مھرباني ڪري مخصوص معلومات لاءِ ليبل تي ختم ٿيڻ جي تاريخ چيڪ ڪريو.
How should I store the NOR-R2 v1.0 product?
The product should be stored according to the storage information provided in Table 1 of the user manual.
Can I use this product for clinical purposes?
No, this product is for research use only.
دستاويز / وسيلا
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Dynamic Biosensors helix cyto Normalization Solution [pdf] استعمال ڪندڙ دستياب helix cyto Normalization Solution, helix cyto, Normalization Solution, Solution |